Anti-Rho antibody [EP487Y] (ab40673) at 1/1000 dilution (purified) + Mouse kidney tissue lysate at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-Rho antibody [EP487Y] (ab40673) at 1/1000 dilution (purified) + C6 cell lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-Rho antibody [EP487Y] (ab40673) at 1/5000 dilution (purified) + HL-60 cell lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-Rho antibody [EP487Y] (ab40673) at 1/1000 dilution (purified) + MDA-MB-435 at 20 µgSecondaryHRP goat anti-rabbit (H+L)

Immunohistochemical staining of paraffin embedded human thyroid carcinoma with purified ab40673 at a working dilution of 1 in 250. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunofluorescence staining of MCF7 cells with purified ab40673 at a working dilution of 1 in 250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, purified ab40673 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500.

Anti-Rho antibody [EP487Y] (ab40673) (unpurified) + 10ug HL60 cell lysate

Unpurified ab40673 at a 1:100 dilution staining Rho in human prostate carcinoma tissue.ab40673 at a 1:100 dilution staining Rho in human prostate carcinoma tissue.