Detection of Human RFC4 by Western Blot of Immunoprecipitate. HeLa whole cell lysate loaded, ab182145 used for blotting immunoprecipitated RFC4 at a 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG used at a 1/1500 dilution.
Immunofluorescent staining of HepG2 cells(right) using anti-RFC4 Alexa Fluor® 555 RabMAB and HepG2 cells (left) using Dapi (blue)
All lanes : Anti-RFC4 antibody [EPR14556] (ab182145) at 1/10000 dilutionLane 1 : Hela lysateLane 2 : HepG2 lysateLane 3 : 293T lysateLane 4 : K562 lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution