Anti-RFC4 antibody [EP14557] - C-terminal

Name	Anti-RFC4 antibody [EP14557] - C-terminal
Supplier	Abcam
Catalog	ab192021
Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EP14557
Applications	ICC/IF ICC/IF IP WB
Species Reactivities	Rat, Human, Mouse
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RFC4 aa 300 to the C-terminus
Description	Rabbit Monoclonal
Gene	RFC4
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Anti-RFC4 antibody [EP14557] - C-terminal (ab192021) at 1/1000 dilution + HeLa cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-RFC4 antibody [EP14557] - C-terminal (ab192021) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : K562 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-RFC4 antibody [EP14557] - C-terminal (ab192021) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : PC12 cell lysateLysates/proteins at 1/20 dilution per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunofluorescence analysis of MCF7 cells labeling RFC4 using ab192021 at 1/100 dilution (7.4 μg/mL). A Goat anti Rabbit IgG (Alexa Fluor488) at 1/200 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permealized with 0.1% triton X-100. Counterstain: DAPI.For the 2 negative cotrol images, ab192021 was used at 1/100 dilution, followed by Goat anti Mouse IgG (Alexa Fluor®594) at 1/500 dilution as secondary antibody.

Western blot analysis of immunoprecipitation pellet from HeLa cell lysate labeling RFC4 with ab192021 at 1/50 dilution (lane 1) or PBS control (lane 2). Secondary staining was with anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG antibody at 1/1500 dilution.