![All lanes : Anti-RENT1/hUPF1 antibody [EPR4681] (ab109363) at 1/10000 dilutionLane 1 : HuT-78 cell lysateLane 2 : Raji cell lysateLane 3 : SH-SY5Y cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.Performed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/22031_RENT1hUPF1-Primary-antibodies-ab109363-1.jpg)
All lanes : Anti-RENT1/hUPF1 antibody [EPR4681] (ab109363) at 1/10000 dilutionLane 1 : HuT-78 cell lysateLane 2 : Raji cell lysateLane 3 : SH-SY5Y cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.Performed under reducing conditions.
ab109363, at 1/100, staining RENT1/hUPF1 in Human kidney tissue by immunohistochemistry.
ab109363, at 1/100, staining RENT1/hUPF1 in Human transitional cell carcinoma tissue by immunohistochemistry.
ab109363, at 1/100, staining RENT1/hUPF1 in HeLa cells by immunofluorescence.
Overlay histogram showing HeLa cells stained with ab109363 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109363, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.