Anti-RASEF antibody [EPR16349]

Name	Anti-RASEF antibody [EPR16349]
Supplier	Abcam
Catalog	ab194827
Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR16349
Applications	ICC/IF ICC/IF FC WB
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RASEF aa 50-150
Description	Rabbit Monoclonal
Gene	RASEF
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-RASEF antibody [EPR16349] (ab194827) at 1/10000 dilutionLane 1 : Mouse brain lysateLane 2 : Mouse heart lysateLane 3 : Mouse spleen lysateLane 4 : Rat brain lysateLane 5 : Rat heart lysateLane 6 : C6 cell lysateLane 7 : RAW 264.7 cell lysateLane 8 : PC12 cell lysateLane 9 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunofluorescent analysis of HeLa cells labeling RASEF with ab194827 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain: DAPI. Cytoplasm staining on HeLa cell line was observed.

Anti-RASEF antibody [EPR16349] (ab194827) at 1/10000 dilution + K562 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-RASEF antibody [EPR16349] (ab194827) at 1/1000 dilutionLane 1 : A549 cell lysateLane 2 : A431 cell lysateLane 3 : Human testis lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Flow cytometry analysis of K562 cells labeling RASEF using ab194827 at 1/50 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody: Blue. Rabbit monoclonal IgG was used as isotype control (Black).