
All lanes : Anti-Rad21 antibody (ab154769) at 1/1000 dilutionLane 1 : K562 whole cell lysateLane 2 : THP1 whole cell lysateLane 3 : HL60 whole cell lysateLane 4 : NCI-H929 whole cell lysateLysates/proteins at 30 µg per lane.

All lanes : Anti-Rad21 antibody (ab154769) at 1/3000 dilutionLane 1 : NIH3T3 whole cell lysateLane 2 : JC whole cell lysateLane 3 : BCL-1 whole cell lysateLysates/proteins at 30 µg per lane.

Immunohistochemical analysis of paraffin embedded Human HBL435 xenograft tissue, labeling Rad21 with ab154769 at 1/750 dilution.

Immunohistochemical analysis of paraffin embedded Mouse C2C12 xenograft tissue, labeling Rad21 with ab154769 at 1/750 dilution.

Immunofluorescent analysis of paraformaldehyde fixed HeLa cells labeling Rad21 with ab154769 at 1/500 dilution. Lower image shows cells costained with Hoechst 33342.