ab99334 at 3µg/ml detecting RAD18 in HeLa whole cell lysate by IP. Lane 1: ab99334 IP at 3µg/mg of lysate Lane 2: IP with an antibody which recognizes an downstream epitope of RAD18 Lane 3: IP control IgG In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded. Subsequent WB detection was performed using an antibody that recognises a downstream epitope of RAD18. Detection: Chemiluminescence with an exposure time of 3 seconds. Two bands represent, but one or both likely represent a post-translationally modified form.