
All lanes : purified at 1/5000 dilutionLane 1 : Mouse brainLane 2 : Rat brainLysates/proteins at 10 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
![All lanes : Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534) at 1/5000 dilution (purified)Lane 1 : MCF7 cell lysateLane 2 : HEK293 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/19621_ab109534-239655-109534-WB-1.jpg)
All lanes : Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534) at 1/5000 dilution (purified)Lane 1 : MCF7 cell lysateLane 2 : HEK293 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Immunohistochemical staining of paraffin embedded human pancreas with purified ab109534 at a working dilution of 1 in 1000. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
![All lanes : Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534) at 1/1000 dilution (unpurified)Lane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 10 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/19623_Rab5-Primary-antibodies-ab109534-1.JPG)
All lanes : Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534) at 1/1000 dilution (unpurified)Lane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 10 µg per lane.

Immunohistochemical staining of Rab5 in paraffin embedded human brain tissue with unpurified ab109534, at 1/100 dilution.