![All lanes : Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [EPR11098] (ab168379) at 1/1000 dilutionLane 1 : Human fetal kidney lysateLane 2 : A549 lysateLane 3 : Jurkat lysateLane 4 : HepG2 lysateLane 5 : HeLa lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/19082_Pyruvate-Dehydrogenase-E1-alpha-subunit-Primary-antibodies-ab168379-1.jpg)
All lanes : Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [EPR11098] (ab168379) at 1/1000 dilutionLane 1 : Human fetal kidney lysateLane 2 : A549 lysateLane 3 : Jurkat lysateLane 4 : HepG2 lysateLane 5 : HeLa lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Pyruvate Dehydrogenase E1-alpha subunit with ab168379 at 1/100 dilution.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Pyruvate Dehydrogenase E1-alpha subunit with ab168379 at 1/100 dilution.

Immunofluorescent analysis of HepG2 cells labeling Pyruvate Dehydrogenase E1-alpha subunit with ab168379 at 1/100 dilution.

Flow cytometric analysis of permeabilized Jurkat cells labeling Pyruvate Dehydrogenase E1-alpha subunit (red) with ab168379 at 1/10 dilution, or a rabbit IgG (negative) (green).

Detection of Pyruvate Dehydrogenase E1-alpha subunit by Western Blot of Immunprecipitate. 293T cell lysate immunoprecipitated using ab168379 at 1/10 dilution; HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.