![All lanes : Anti-PUF60 antibody [GT677] (ab184538) at 1/5000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLane 3 : HeLa whole cell lysateLane 4 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/18700_ab184538-215148-ab184538Wb.jpg)
All lanes : Anti-PUF60 antibody [GT677] (ab184538) at 1/5000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLane 3 : HeLa whole cell lysateLane 4 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.
![Anti-PUF60 antibody [GT677] (ab184538) at 1/1000 dilution + Mouse brain lysate at 50 µg](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/18701_ab184538-215147-ab184538WBb.jpg)
Anti-PUF60 antibody [GT677] (ab184538) at 1/1000 dilution + Mouse brain lysate at 50 µg
![Anti-PUF60 antibody [GT677] (ab184538) at 1/1000 dilution + Rat brain lysate at 50 µg](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/18702_ab184538-215146-ab184538WBc.jpg)
Anti-PUF60 antibody [GT677] (ab184538) at 1/1000 dilution + Rat brain lysate at 50 µg

Immunofluorescent analysis of A431 cells (fixed in 4% paraformaldehyde, RT for 15 mins) labeling PUF60 with ab184538 at 1/500 dilution (Green). Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded AGS gastric carcinoma xenograft labeling PUF60 with ab184538 at 1/100 dilution.