![Anti-PSMA antibody [EPR6253] (ab133579) at 1/100000 dilution (Purified) + LNCaP lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L)](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/17786_ab133579-236627-133579-WB.jpg)
Anti-PSMA antibody [EPR6253] (ab133579) at 1/100000 dilution (Purified) + LNCaP lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L)

Immunohistochemical staining of paraffin embedded human prostate cancer with purified ab133579 at a working dilution of 1 in 300. HRP polymer for rabbit IgG was used as the secondary antibody, and the sample was counter stained with hematoxylin.

Immunohistochemical staining of paraffin embedded rat kidney with purified ab133579 at a working dilution of 1 in 300. HRP polymer for rabbit IgG was used as the secondary antibody, and the sample was counter stained with hematoxylin.

Immunohistochemical staining of paraffin embedded mouse kidney with purified ab133579 at a working dilution of 1 in 300. HRP polymer for rabbit IgG was used as the secondary antibody, and the sample was counter stained with hematoxylin.

Primary: ab133579, 1/50 dilutionDilution buffer: NFDM/TBST, 5%Sample: LNCaPSecondary: HRP goat anti-rabbit (H+L), 1/1000 dilution
![Anti-PSMA antibody [EPR6253] (ab133579) at 1/50000 dilution (Unpurified) + LNCaP cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/17791_PSMA-Primary-antibodies-ab133579-2.jpg)
Anti-PSMA antibody [EPR6253] (ab133579) at 1/50000 dilution (Unpurified) + LNCaP cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Unpurified ab133579 showing positive staining in Prostatic carcinoma tissue.

Unpurified ab133579 showing positive staining in Normal prostate tissue.

Unpurified ab133579 showing negative staining in Thyroid gland carcinoma tissue.

Unpurified ab133579 showing negative staining in Normal colon tissue.

Unpurified ab133579 showing negative staining in Breast carcinoma tissue.

Unpurified ab133579 showing negative staining in Normal spleen tissue.