Lane 1 : Anti-PSD95 (phospho S295) antibody (ab16495) at 0.3 µg/mlLane 2 : Anti-PSD95 (phospho S295) antibody (ab16495) at 1 µg/mlLanes 3 - 5 : Anti-PSD95 (phospho S295) antibody (ab16495) at 3 µg/mlLane 6 : Anti-PSD95 antibody (ab12093) at 0.3 µg/mlLane 1 : mouse hippocampus lysateLane 2 : mouse hippocampus lysateLane 3 : mouse hippocampus lysateLane 4 : mouse hippocampus lysate with Mouse PSD95 (phospho S295) peptide (ab17222) at 3 µg/mlLane 5 : mouse hippocampus lysate with Mouse PSD95 peptide (ab17223) at 3 µg/mlLane 6 : mouse hippocampus lysate
Lane 1 - Input lane, 25-50µg mouse forebrain brain lysate probed with ab16495 PSD95 (phospho S295) antibody (1µg/ml)Lane 2 - IP lane, ~500µg mouse forebrain brain lysate probed with ab16495 PSD95 (phospho S295) antibody (1µg/ml)The IP lane (2) shows an enrichment of PSD95 (phospho S295) at the expected band size (~105kDa).
![Immunohistochemistical detection of PSD95 (phospho S295) using antibody ab16495 on PFA perfusion-fixed mouse brain sections (30 microns). Primary antibody ab16495 was diluted at 1/300 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor 488 (1/1000). The image show immunostaining obtained in the mouse cerebellum using this antibody using direct fluorescence. The antibody stained granular cerebellar cells. Image [A] shows the staining obtained using the antibody, whereas image [B] is the staining captured with the same setup of acquisition for the negative control (lack of primary antibody). The arrows show the location of purkinje cells.See Abreview](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/17642_PSD95-Primary-antibodies-ab16495-3.jpg)
Immunohistochemistical detection of PSD95 (phospho S295) using antibody ab16495 on PFA perfusion-fixed mouse brain sections (30 microns). Primary antibody ab16495 was diluted at 1/300 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor 488 (1/1000). The image show immunostaining obtained in the mouse cerebellum using this antibody using direct fluorescence. The antibody stained granular cerebellar cells. Image [A] shows the staining obtained using the antibody, whereas image [B] is the staining captured with the same setup of acquisition for the negative control (lack of primary antibody). The arrows show the location of purkinje cells.See Abreview