![All lanes : Anti-PRPF8 antibody [EPR15229] - C-terminal (ab185547) at 1/20000 dilutionLane 1 : K562 cell lysateLane 2 : 293T cell lysateLane 3 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/17356_ab185547-219624-ab1855471.jpg)
All lanes : Anti-PRPF8 antibody [EPR15229] - C-terminal (ab185547) at 1/20000 dilutionLane 1 : K562 cell lysateLane 2 : 293T cell lysateLane 3 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin embedded Human cervix carcinoma tissue sections labeling PRPF8 using ab185547 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Immunofluorescent analysis of formaldehyde fixed HeLa cells labeling PRPF8 using ab185547 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

Flow Cytometry analysis of K562 cells labeling PRPF8 using ab185547 at a 1/190 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.