ab172060 staining Protein C inhibitor in Human brainstem (pons) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 2 hours at 20°C; antigen retrieval was by heat mediation in a citrate buffer, pH6. Samples were incubated with primary antibody (1/200 in 2% serum, 0.5% Triton X-100 in PBS) for 20 hours at 20°C. A Biotin-conjugated Sheep anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.See Abreview