![All lanes : Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab184553) at 1/20000 dilutionLane 1 : HepG2 cell lysateLane 2 : 293 cell lysateLane 3 : Jurkat cell lysateLane 4 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/17037_ab184553-216790-ab184553WB.jpg)
All lanes : Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab184553) at 1/20000 dilutionLane 1 : HepG2 cell lysateLane 2 : 293 cell lysateLane 3 : Jurkat cell lysateLane 4 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Protective protein/Cathepsin A (PPCA) with ab184553 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Protective protein/Cathepsin A (PPCA) with ab184553 at 1/140 dilution (red) compared with a Rabbit monoclonal IgG isotype control (green), followed by Goat anti-rabbit IgG (FITC) secondary antibody at 1/150 dilution.