To detect Murine RELM-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine RELM-beta (38-102) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine RELM-beta.
To detect Murine RELM-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. when used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELM-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Murine RELM-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. when used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELM-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained 4% PFA, paraffin-embedded sections of murine cecum tissue (with positive goblet cells of a Trichuris muris infected mouse). The primary antibody was incubated at a concentration of 0.33 ng/mL overnight at 4˚C and the secondary antibody was a cyanine-2 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM Citric Acid was used.