All lanes : Anti-PPP1R11 antibody [EPR11965] (ab171960) at 1/1000 dilutionLane 1 : Human testis lysateLane 2 : HeLa cell lysateLane 3 : Fetal brain lysateLane 4 : NIH: OVCAR-3 lysateLane 5 : MDA-MB-435 lysateLysates/proteins at 10 µg per lane.
Flow cytometric analysis of permeabilized HeLa cells labeling PPP1R11 with ab171960 at 1/10 dilution (red) or a Rabbit IgG (negative) (green).
Western blot analysis on immunoprecipitation pellet from Human testis lysate (1) labeling PPP1R11 with ab171960 at 1/10 dilution or 1X PBS (2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.