All lanes : Anti-PPM1J antibody (ab169362) at 1/500 dilutionLane 1 : PPM1J transfected 293T cell lysateLane 2 : Non transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat anti-rabbit IgG HRP at 1/7500 dilution
Anti-PPM1J antibody (ab169362) at 1/500 dilution + mouse kidney tissue lysate at 50 µgSecondaryGoat anti-rabbit IgG HRP at 1/7500 dilution
Immunoprecipitation of PPM1J from PPM1J transfected lysate, using ab169362 and Protein A Magnetic Beads. An anti-PPM1J purified mouse polyclonal antibody was used for immunoblotting.