All lanes : Anti-POLR2E antibody [3B5] (ab119316) at 1/500 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY POLR2E cDNALysates/proteins at 5 µg per lane.
All lanes : Anti-POLR2E antibody [3B5] (ab119316) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : SVT2 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.
ab119316, at 1/150 dilution, staining POLR2E in paraffin-embedded Human breast tissue by Immunohistochemistry.
ab119316, at 1/150 dilution, staining POLR2E in paraffin-embedded Human lung carcinoma tissue by Immunohistochemistry.
ab119316, at 1/150 dilution, staining POLR2E in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
ab119316, at 1/150 dilution, staining POLR2E in paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
ab119316, at 1/150 dilution, staining POLR2E in paraffin-embedded Human prostate carcinoma tissue by Immunohistochemistry.
ab119316, at 1/150 dilution, staining POLR2E in paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
ab119316, at 1/150 dilution, staining POLR2E in paraffin-embedded Human lymphoma tissue by Immunohistochemistry.
ab119316, at 1/150 dilution, staining POLR2E in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
HEK293T cells transfected with either POLR2E overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab119316 and then analyzed by flow cytometry
Flow cytometric Analysis of Jurkat cells, using ab119316 (Red), compared to a nonspecific negative control antibody,(Blue).