Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human osteosarcoma tissue labelling PKM2 with ab131021 at 1/1000 (1µg/ml). Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling PKM2 with ab131021 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-PKM2 antibody (ab131021) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : 293T whole cell lysate at 50 µgLane 4 : Jurkat whole cell lysate at 50 µgdeveloped using the ECL technique
Detection of PKM2 by Western Blot of Immunprecipitate. ab131021 at 0.4µg/ml staining PKM2 in HeLa whole cell lysate immunoprecipitated using ab131021 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds.