ICC/IF image of ab23707 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23707, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PINK1 antibody (ab23707) at 1/1000 dilution + Whole cell lysate prepared from Jurkat cells at 100000 cellsSecondaryDonkey anti-rabbit IgG conjugated to HRP at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 66 kDa (why is the actual band size different from the predicted?)Additional bands at : 100 kDa,35 kDa. We are unsure as to the identity of these extra bands.Exposure time : 10 secondsThis image is courtesy of an anonymous AbreviewSee Abreview