Anti-PFKFB3 antibody [EPR12594]

Name	Anti-PFKFB3 antibody [EPR12594]
Supplier	Abcam
Catalog	ab181861
Prices	$384.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR12594
Applications	WB IP ICC/IF ICC/IF IHC-P
Species Reactivities	Mouse, Rat, Human
Antigen	Recombinant fragment within Human PFKFB3 aa 300 to the C-terminus
Blocking Peptide	Human PFKFB3 protein fragment
Description	Rabbit Monoclonal
Gene	PFKFB1
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-PFKFB3 antibody [EPR12594] (ab181861) at 1/20000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling PFKFB3 with ab181861 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Immunofluorescent analysis of acetone-fixed HeLa cells labeling PFKFB3 with ab181861 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Counter stained with Dapi (blue).

Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling PFKFB3 with ab181861 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Counter stained with Dapi (blue).

Western blot analysis of PFKFB3 in HeLa cell lysate immunoprecipitated using ab181861 at 1/50 dilution (Lane 1). Lane 2: Negative control.Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.