ab2426 staining PCNA in murine skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using TRIS EDTA pH 8.2. Samples were then incubated with the primary antibody at a 1/2000 dilution for 1 hour at 37°C. An undiluted HRP-conjugated rabbit polyclonal was used as secondary antibody.See Abreview

ab2426 staining PCNA in rat tumor tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/500 in PBS) for 12 hours at 4°C. A HRP-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.See Abreview

ab2426 was used for immunohistochemistry, at a 1:500 dilution in hamster neural tissue, identified with a polyclonal secondary and DAB detection kit. Review by Kevin Bath submitted 18 June 2004

ab2426 at 1/200 dilution staining rat skeletal muscle satellite stem cells by ICC/IF.Cultured rat skeletal muscle satellite stem cells (SKMB) were 2% paraformaldehyde fixed for 15 minutes and then permealized with Triton-X100 prior to incubation with ab2426 overnight at 4°C. A donkey anti-rabbit Alexa-Fluor ® 488 (ab150073) antibody was used as the secondary.The image shows DAPI (blue-nuclear stain, upper left panel), PCNA (Green, upper right panel, showing nuclear localization in actively dividing cells), same SKMB cells -DIC (phase) image (lower left panel) and superimpose fluorescence image (lower right panel).See Abreview

ab2426 staining PCNA in murine epithelial tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Wound tissue sections (0.5 µm) were cut using a microtome and collected on slides. Sections were then de-waxed in xylene and rehydrated by successive immersion in descending concentrations of alcohol. The sections were then subjected for immunofluorescence staining. Briefly, tissue sections were blocked by incubated with 5% donkey serum (ab7475) for 1 hour and washed with phosphate-buffered saline (PBS). Sections were then incubated with ab2426 at a 1/500 for 1 hour at room temperature under humidified conditions. After primary antibody incubation, sections were washed with PBS and incubated with appropriate fluorescent secondary antibodies for 1 hour at room temperature. Sections were then washed with PBS, mounted with mounting medium containing DAPI.

All lanes : Anti-PCNA antibody (ab2426) at 1/200 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryIRDye 680 conjugated Goat anti-rabbit IgG (H&L) at 1/15000 dilution

Anti-PCNA antibody (ab2426) at 1/1000 dilution + 293T whole cell lysate at 70 µgSecondaryHRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

All lanes : Anti-PCNA antibody (ab2426) at 1/300 dilutionLane 1 : Whole tissue lysate prepared from mouse heartLane 2 : Whole tissue lysate prepared from mouse heartLane 3 : Whole tissue lysate prepared from mouse heartLane 4 : Whole tissue lysate prepared from mouse heartLysates/proteins at 50 µg per lane.SecondaryHRP-conjugated pig anti-rabbit polyclonal at 1/3000 dilutiondeveloped using the ECL technique