![All lanes : Anti-PBK (phospho T9) antibody [EPR2475(N)] (ab184953) at 1/5000 dilutionLane 1 : Hela lysate treated with NocodazoleLane 2 : Hela lysate untreatedLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/7914_ab184953-217645-ab184953.jpg)
All lanes : Anti-PBK (phospho T9) antibody [EPR2475(N)] (ab184953) at 1/5000 dilutionLane 1 : Hela lysate treated with NocodazoleLane 2 : Hela lysate untreatedLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling PBK (phospho T9) with ab184953 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling PBK (phospho T9) with ab184953 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin

Flow cytometric analysis of 2% paraformaldehyde-fixed, permeabilized HeLa cells treated with Nocodazole labeling PBK (phospho T9) with ab184953 at 1/100 dilution (red) compared to untreated HeLa cells (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution. Nocodazole-treated HeLa cells labeled using ab184953 preincubated with phospho PBK (phospho T9) peptide (blue) or non phosphopeptide (orange).