
All lanes : Anti-PAX6 antibody (ab154253) at 1/1000 dilutionLane 1 : A431 whole cell lysateLane 2 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.

Immunoprecipitation of PAX6 using 2 µg of ab154253, and detected using ab154253 at 1/1000 dilution. Lane 1: 1000 µg 293T whole cell lysate. Lane 2: 50 µg 293T whole cell lysate Lane 3: 2 µg preimmune control Rabbit IgG10% SDS-PAGE

Immunofluorescent analysis of paraformaldehyde-fixed A549 cells labeling PAX6 with ab154253 at 1/200 dilution. The image in the lower panel was merged with DNA probe.

Immunofluorescent analysis of paraformaldehyde-fixed 293T cells labeling PAX6 with ab154253 at 1/250 dilution.