![All lanes : Anti-PAX5 antibody [EPR3730(2)] (ab109443) at 1/10000 dilutionLane 1 : Ramos lysateLane 2 : Human tonsil lysateLane 3 : Daudi lysates Lysates/proteins at 10 µg per lane.SecondaryStandard HRP labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/7718_PAX5-Primary-antibodies-ab109443-1.jpg)
All lanes : Anti-PAX5 antibody [EPR3730(2)] (ab109443) at 1/10000 dilutionLane 1 : Ramos lysateLane 2 : Human tonsil lysateLane 3 : Daudi lysates Lysates/proteins at 10 µg per lane.SecondaryStandard HRP labelled goat anti-rabbit at 1/2000 dilution
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab109443
ab109443 showing positive staining in Hodgkin's lymphoma tissue.
ab109443 showing negative staining in Normal kidney tissue.
ab109443 showing positive staining in Normal spleen tissue.
ab109443 showing positive staining in Diffuse B-cell lymphoma tissue.
Overlay histogram showing Ramos cells stained with ab109443 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109443, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD