
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling PARP4 with ab86066 at 1/200 (1µg/ml). Detection: DAB.

All lanes : Anti-PARP4 antibody (ab86066) at 0.04 µg/mlLane 1 : Hela whole cell lysate at 50 µgLane 2 : Hela whole cell lysate at 15 µgLane 3 : Hela whole cell lysate at 5 µgdeveloped using the ECL technique

1 mg whole cell lysate from HeLa cells was immunoprecipitated using 3 µg of ab86066. 20% of IP was loaded in each lane and probed with ab86066 at 0.4 µg/ml (lane 1) or with a control IgG (lane 2). Detection: chemiluminescence with an exposure time of 30 seconds.