Anti-pan-AKT antibody
| Name | Anti-pan-AKT antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab18785 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F WB IP |
| Species Reactivities | Mouse, Rat, Bovine, Human |
| Antigen | Synthetic peptide corresponding to residues surrounding aa 80 of Akt (N terminal)(Human) (Peptide available as ab53312 |
| Blocking Peptide | AKT1 peptide |
| Description | Rabbit Polyclonal |
| Gene | AKT1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-pan-AKT antibody (ab18785) + Akt expression in NIH3T3 cell lysate
ICC/IF image of ab18785 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18785, ug/ml) overnight at +4oC. The secondary antibody (green)was Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.