ICC/IF image of ab2433 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab2433, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was a goat anti-rabbit Alexa Fluor® 488 (IgG H+L)ab150077) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).