Anti-P2RX7 antibody [EPR4723] (ab109246) at 1/2600 dilution (purified) + Jurkat cell lysate at 1/1000 dilutionSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-P2RX7 antibody [EPR4723] (ab109246) at 1/80 dilution (unpurified)Lane 1 : Mouse brain tissue lysateLane 2 : Rat brain tissue lysateLysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-P2RX7 antibody [EPR4723] (ab109246) at 1/1000 dilution (purified)Lane 1 : Mouse brain tissue lysateLane 2 : Rat brain tissue lysateLysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-P2RX7 antibody [EPR4723] (ab109246) at 1/1000 dilution (unpurified)Lane 1 : Jurkat cell lysateLane 2 : A431 cell lysateLane 3 : HeLa cell lysateLane 4 : Human fetal kidney tissue lysateLysates/proteins at 10 µg per lane.

Immunocytochemsitry/Immunofluorescence analysis of U87-MG cells labelling P2RX7 (green) with unpurified ab109246 at 1/20. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

Immunocytochemsitry/Immunofluorescence analysis of U87-MG cells labelling P2RX7 (green) with purified ab109246 at 1/300. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling P2RX7 (red) with unpurified ab109246 at 1/100.

Overlay histogram showing HeLa cells stained with unpurified ab109246 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109246, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Flow cytometry analysis of HeLa cells labelling P2RX7 with unpurified ab109246 at 1/20 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.