Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y]
| Name | Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab62364 |
| Prices | $403.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP233(2)Y |
| Applications | WB IP IHC-P |
| Species Reactivities | Mouse, Rat, Human, Monkey |
| Antigen | A synthetic phosphopeptide corresponding to residues surrounding Serine 10 of human p27 KIP 1 |
| Description | Rabbit Monoclonal |
| Gene | CDKN1B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Immunohistochemistical detection of p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364) on formaldehyde fixed paraffin-embedded monkey cerebellum sections. Antigen retrieval step: Heat mediated. Buffer: Citric acid solution. Permeabilization: None. Blocking step: 1% BSA for 10 mins. Primary antibody incubated at 1/1500 for 2 hours @ 21°C in TBS/BSA/azide. Secondary antibody Name: anti rabbit IgG conjugated to biotin (1/200).See Abreview](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/5389_p27-KIP-1-Primary-antibodies-ab62364-8.jpg)
Immunohistochemistical detection of p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364) on formaldehyde fixed paraffin-embedded monkey cerebellum sections. Antigen retrieval step: Heat mediated. Buffer: Citric acid solution. Permeabilization: None. Blocking step: 1% BSA for 10 mins. Primary antibody incubated at 1/1500 for 2 hours @ 21°C in TBS/BSA/azide. Secondary antibody Name: anti rabbit IgG conjugated to biotin (1/200).See Abreview
ICC/IF image of p27 KIP 1 (phospho S10) staining using ab62364 on Cor1 cells. Cells were fixed for 20 mins using 4% Formalin in PBS. After TBS washes, cells were then permeabilised/blocked simultaneously using antibody diluent before applying primary Ab.Some customers have sucessfully used the antibody in ICC/IF. Please see the abreview for the experimental conditions for the antibody staining. See Abreview
![All lanes : Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364) at 1/10000 dilutionLane 1 : MCF-7 cell lysate (untreated)Lane 2 : MCF-7 cell lysate treated with APLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP labeled at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/5391_ab62364wb.gif)
All lanes : Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364) at 1/10000 dilutionLane 1 : MCF-7 cell lysate (untreated)Lane 2 : MCF-7 cell lysate treated with APLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP labeled at 1/2000 dilution
![Immunohistochemistical detection of p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364) on formaldehyde-fixed paraffin-embedded human tonsil sections. Antigen retrieval step: Heat mediated. Buffer Used: Citric acid. Permeabilization: None. Blocking step: 1% BSA for 10 mins @ 21°C. Primary antibody incubated at 1/500 for 2 hours @ 21°C in TBS/BSA/azide. Notes: A micropolymer HRP kit was used to obtain this intensity of immunostaining as I could not achieve a similar intensity of positivity on human tissues, under the same conditions (compare with results on rat/mouse/marmoset sections, using a std ABCpx detection system). There is one obvious metaphase nucleus in the Germinal centre of the submitted image: it is not positive.See Abreview](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/5392_p27-KIP-1-Primary-antibodies-ab62364-10.jpg)
Immunohistochemistical detection of p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364) on formaldehyde-fixed paraffin-embedded human tonsil sections. Antigen retrieval step: Heat mediated. Buffer Used: Citric acid. Permeabilization: None. Blocking step: 1% BSA for 10 mins @ 21°C. Primary antibody incubated at 1/500 for 2 hours @ 21°C in TBS/BSA/azide. Notes: A micropolymer HRP kit was used to obtain this intensity of immunostaining as I could not achieve a similar intensity of positivity on human tissues, under the same conditions (compare with results on rat/mouse/marmoset sections, using a std ABCpx detection system). There is one obvious metaphase nucleus in the Germinal centre of the submitted image: it is not positive.See Abreview
ab62364 at 1/100 dilution staining p27 KIP 1 in human ovarian carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
ab62364 at 1/100 dilution staining p27 KIP 1 in human tonsils by Immunohistochemistry, Paraffin-embedded tissue.
Product References
TRIP6 regulates p27 KIP1 to promote tumorigenesis. - TRIP6 regulates p27 KIP1 to promote tumorigenesis.
Lin VT, Lin VY, Lai YJ, Chen CS, Liu K, Lin WC, Lin FT. Mol Cell Biol. 2013 Apr;33(7):1394-409.
Cdc6 protein activates p27KIP1-bound Cdk2 protein only after the bound p27 - Cdc6 protein activates p27KIP1-bound Cdk2 protein only after the bound p27
Uranbileg B, Yamamoto H, Park JH, Mohanty AR, Arakawa-Takeuchi S, Jinno S, Okayama H. J Biol Chem. 2012 Feb 24;287(9):6275-83.
Successive phosphorylation of p27(KIP1) protein at serine-10 and C terminus - Successive phosphorylation of p27(KIP1) protein at serine-10 and C terminus
Mohanty AR, Kan Q, Srivastava S, Uranbileg B, Arakawa-Takeuchi S, Fujita N, Okayama H. J Biol Chem. 2012 Jun 22;287(26):21757-64.
Cell cycle arrest in node cells governs ciliogenesis at the node to break - Cell cycle arrest in node cells governs ciliogenesis at the node to break
Komatsu Y, Kaartinen V, Mishina Y. Development. 2011 Sep;138(18):3915-20.
Targeted deletion of Capn4 in cells of the chondrocyte lineage impairs - Targeted deletion of Capn4 in cells of the chondrocyte lineage impairs
Kashiwagi A, Schipani E, Fein MJ, Greer PA, Shimada M. Mol Cell Biol. 2010 Jun;30(11):2799-810.
CCK1 and CCK2 receptors are expressed on pancreatic stellate cells and induce - CCK1 and CCK2 receptors are expressed on pancreatic stellate cells and induce
Berna MJ, Seiz O, Nast JF, Benten D, Blaker M, Koch J, Lohse AW, Pace A. J Biol Chem. 2010 Dec 10;285(50):38905-14.
Phosphorylation of p27Kip1 by Epstein-Barr virus protein kinase induces its - Phosphorylation of p27Kip1 by Epstein-Barr virus protein kinase induces its
Iwahori S, Murata T, Kudoh A, Sato Y, Nakayama S, Isomura H, Kanda T, Tsurumi T. J Biol Chem. 2009 Jul 10;284(28):18923-31.