Overlay histogram showing SH-SY5Y cells stained with ab68151 (red line) at 1/280 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (black line) was rabbit monoclonal IgG used under the same conditions. Cells also incubated without primary antibody and secondary antibody (blue line)
![Anti-NGF antibody [EP1318Y] (ab68151) at 1/1000 dilution + Rat brain lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/173_ab68151-240307-68151-WB2.jpg)
Anti-NGF antibody [EP1318Y] (ab68151) at 1/1000 dilution + Rat brain lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution
![Anti-NGF antibody [EP1318Y] (ab68151) at 1/1000 dilution + Human fetal brain lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/174_ab68151-240304-68151-WB1.jpg)
Anti-NGF antibody [EP1318Y] (ab68151) at 1/1000 dilution + Human fetal brain lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution
![All lanes : Anti-NGF antibody [EP1318Y] (ab68151) at 1/5000 dilution (unpurified)Lane 1 : 10ng human pro-NGF recombinant proteinLane 2 : 5ng human pro-NGF recombinant proteinLane 3 : 1ng human pro-NGF recombinant proteinLane 4 : 0.5ng human pro-NGF recombinant proteinSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/175_ab68151.jpg)
All lanes : Anti-NGF antibody [EP1318Y] (ab68151) at 1/5000 dilution (unpurified)Lane 1 : 10ng human pro-NGF recombinant proteinLane 2 : 5ng human pro-NGF recombinant proteinLane 3 : 1ng human pro-NGF recombinant proteinLane 4 : 0.5ng human pro-NGF recombinant proteinSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution