Anti-NG2 antibody

Name	Anti-NG2 antibody
Supplier	Abcam
Catalog	ab104535
Prices	$385.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Applications	WB IHC-P IHC-F ICC/IF ICC/IF
Species Reactivities	Human, Mouse, Rat
Antigen	A synthetic peptide from the extracellular domain of Human NG2 conjugated to an immunogenic carrier protein
Description	Rabbit Polyclonal
Gene	CSPG4
Conjugate	Unconjugated
Supplier Page	Shop

Product images

ab104535 at 1/1000 dilution staining NG2 in Human breast cancer by Immunohistochemistry, Paraffin-embedded tissue.

ICC/IF image of ab104535 stained Malme-3M cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104535, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

T lymphocytes redirected against the chondroitin sulfate proteoglycan-4 control - T lymphocytes redirected against the chondroitin sulfate proteoglycan-4 control

Geldres C, Savoldo B, Hoyos V, Caruana I, Zhang M, Yvon E, Del Vecchio M, Creighton CJ, Ittmann M, Ferrone S, Dotti G. Clin Cancer Res. 2014 Feb 15;20(4):962-71.