All lanes : Anti-NFAT2 antibody (ab134469) at 1 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.