Anti-NeuroD2 antibody [EPR5135] - ChIP Grade

Name	Anti-NeuroD2 antibody [EPR5135] - ChIP Grade
Supplier	Abcam
Catalog	ab109406
Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR5135
Applications	ChIP WB IHC-P
Species Reactivities	Mouse, Rat, Human
Antigen	A synthetic peptide corresponding to residues in Human NeuroD2
Description	Rabbit Monoclonal
Gene	NEUROD2
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-NeuroD2 antibody [EPR5135] - ChIP Grade (ab109406) at 1/1000 dilutionLane 1 : Human cerebellum lysateLane 2 : Human hippocampus lysateLane 3 : Human fetal brain lysatesLysates/proteins at 10 µg per lane.

Immunohistochemical analysis of paraffin-embedded Human brain tissue using 1/250 ab109406

ChIP analysis using ab109406 binding NeuroD2 in cerebral cortex tissue lysate from postnatal day 0 mouse. Cells were cross-linked for 10 minutes with 1% formaldehyde. Samples were incubated with primary antibody (1/200) for 2 hours at 4°C.Positive control: Known target of NeuroD2, which is the promoter regions of Nhlh2 gene.Primer sequence for PCR was:Nhlh2_F CTCACGAACTTCACCCGCACNhlh2_R AAATTGACTCCTCGGCCCTCNegative Control: ChIP performed with unrelated antibody (anti-GFP antibody).See Abreview

Product References

Contribution of microRNA-1275 to Claudin11 protein suppression via a - Contribution of microRNA-1275 to Claudin11 protein suppression via a

Katsushima K, Shinjo K, Natsume A, Ohka F, Fujii M, Osada H, Sekido Y, Kondo Y. J Biol Chem. 2012 Aug 10;287(33):27396-406.