![All lanes : Anti-NCF1 antibody [EPR13134] (ab179457) at 1/1000 dilutionLane 1 : Raji cell lysateLane 2 : Ramos cell lysateLane 3 : THP1 cell lysateLysates/proteins at 10 µg per lane.SecondaryStandard HRP labeled goat anti-rabbit at 1/2000 dilutiondeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/27990_ab179457-207492-ab179457wb.jpg)
All lanes : Anti-NCF1 antibody [EPR13134] (ab179457) at 1/1000 dilutionLane 1 : Raji cell lysateLane 2 : Ramos cell lysateLane 3 : THP1 cell lysateLysates/proteins at 10 µg per lane.SecondaryStandard HRP labeled goat anti-rabbit at 1/2000 dilutiondeveloped using the ECL technique
Flow cytometry analysis of permeabilized Raji cells labeling NCF1 (red) with ab179457 at a 1/10 dilution, or negative control rabbit IgG (green)
Western blot analysis on Immunoprecipitation pellet from either 1) Raji cell lysate, or 2) 1xPBS (negative control); showing NCF1, using ab179457 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.