Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal

Name	Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal
Supplier	Abcam
Catalog	ab177954
Prices	$385.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR12716
Applications	WB FC
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Natriuretic Peptide Receptor C aa 500 to the C-terminus (Cysteine residue)
Description	Rabbit Monoclonal
Gene	NPR3
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954) at 1/1000 dilution (purified)Lane 1 : Human tissue lysateLane 2 : Human tissue lysateLysates/proteins at 1/10 dilution per lane.SecondaryHRP anti-rabbit, specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954) at 1/7000 dilution (purified)Lane 1 : Mouse kidney tissue lysateLane 2 : Rat kidney tissue lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

All lanes : Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954) at 1/1000 dilution (unpurified)Lane 1 : Mouse kidney tissue lysateLane 2 : Rat kidney tissue lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Overlay histogram showing HeLa cells fixed in 2% PFA and stained with purified ab177954 at a dilution of 1 in 270 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.

All lanes : Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954) at 1/1000 dilution (unpurified)Lane 1 : Human fetal kidney lysateLane 2 : Human placenta lysateLane 3 : HeLa lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Flow Cytometric analysis of permeabilized HeLa cells labeling Natriuretic Peptide Receptor C with unpurified ab177954 at 1/100 dilution (red) or a rabbit IgG (negative) (green).