![Anti-NARS antibody [NARS2G5] (ab53473) at 1/100 dilution + NIH 3T3 whole cell lysate at 50 µgSecondaryanti-mouse IgG at 1/2500 dilutiondeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/27668_ab53473wbnih.gif)
Anti-NARS antibody [NARS2G5] (ab53473) at 1/100 dilution + NIH 3T3 whole cell lysate at 50 µgSecondaryanti-mouse IgG at 1/2500 dilutiondeveloped using the ECL technique

ab53473 at 5ug used to immunoprecipitate RIPA lysate of HT-1080 cells (500µg). Western blot used ab53473 at 1/50 dilution with anti-mouse IgG at 1/2500 as secondary.

ab53473 at 1/10 dilution staining NARS in HT-1080 cells, by Immunocytochemistry. AlexaFluor®488 Goat Anti-mouse IgG at 1/200 used as secondary.
![Anti-NARS antibody [NARS2G5] (ab53473) at 1/50 dilution + HT-1080 whole cell lysate at 25 µgSecondaryanti-mouse IgG at 1/2500 dilutiondeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/27671_ab53473wbht.gif)
Anti-NARS antibody [NARS2G5] (ab53473) at 1/50 dilution + HT-1080 whole cell lysate at 25 µgSecondaryanti-mouse IgG at 1/2500 dilutiondeveloped using the ECL technique