Anti-N Cadherin antibody [8C11]

Name	Anti-N Cadherin antibody [8C11]
Supplier	Abcam
Catalog	ab19348
Prices	$384.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone	8C11
Applications	WB ICC/IF ICC/IF IP
Species Reactivities	Rabbit, Hamster, Human, Bird
Antigen	Fusion protein corresponding to N Cadherin (extracellular)
Description	Mouse Monoclonal
Gene	CDH2
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Lane 1 : Anti-N Cadherin antibody [8C11] (ab19348) at 1/1000 dilutionLanes 2 - 3 : Anti-N Cadherin antibody [8C11] (ab19348) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : HUVEC cell lysateLane 3 : C2C12 cell lysateLysates/proteins at 25 µg per lane.Observed band size : 140 kDa (why is the actual band size different from the predicted?)

Immunofluorescence analysis of ARPE-19 cells, staining N Cadherin (green) with ab19348, at 1/20 dilution. ARPE-19 monolayer cultures were fixed in paraformaldehyde, permeabilized with 0.2% Triton X-100 for 15 min and blocked with 2% BSA for 30 min. Samples were incubated with primary antibody for 16 hours at 4°C before incubation with an AlexaFluor®488-conjugated donkey anti-mouse secondary IgG for 60 min.

Product References

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Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast - Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast

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Shintani Y, Okimura A, Sato K, Nakagiri T, Kadota Y, Inoue M, Sawabata N, Minami M, Ikeda N, Kawahara K, Matsumoto T, Matsuura N, Ohta M, Okumura M. Ann Thorac Surg. 2011 Nov;92(5):1794-804; discussion 1804. doi:

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Ke XS, Qu Y, Goldfinger N, Rostad K, Hovland R, Akslen LA, Rotter V, Oyan AM, Kalland KH. PLoS One. 2008;3(10):e3368.

N-cadherin-catenin complexes form prior to cleavage of the proregion and - N-cadherin-catenin complexes form prior to cleavage of the proregion and

Wahl JK 3rd, Kim YJ, Cullen JM, Johnson KR, Wheelock MJ. J Biol Chem. 2003 May 9;278(19):17269-76. Epub 2003 Feb 25.