Anti-N Cadherin antibody
| Name | Anti-N Cadherin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab76057 |
| Prices | $403.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP ELISA ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Sheep, Human, Rat, Chicken, Chimpanzee |
| Antigen | Unphosphorylated synthetic peptide: ERPIHAEPQYPVRS , corresponding to amino acids 811-824 of Human N Cadherin (coupled to a carrier protein) |
| Description | Rabbit Polyclonal |
| Gene | CDH2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab76057 staining N Cadherin in human BicR19 cells by Flow Cytometry. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/100) for 30 minutes. An undiluted Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Gating Strategy: Oral epithelial cells.See Abreview
All lanes : Anti-N Cadherin antibody (ab76057) at 1/1000 dilutionLane 1 : Human endothelial cells untreatedLane 2 : Human endothelial cells treated with pervanadate (1 mM) for 30 min
ab76057 at 1:100, staining differentiated embryonic stem cells by ICC/IFSee Abreview
All lanes : Anti-N Cadherin antibody (ab76057) at 1/1000 dilutionLane 1 : MDA-MB-231 cells (Negative Control)Lane 2 : U2OS cells (Positive Control)Lysates/proteins at 200000 cells per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of N Cadherin staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76057, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
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Kim DH, Kim HR, Choi EJ, Kim DY, Kim KK, Kim BS, Park JW, Lee BJ. PLoS One. 2014 Jan 21;9(1):e85898.
SWI/SNF chromatin-remodeling factor Smarcd3/Baf60c controls - SWI/SNF chromatin-remodeling factor Smarcd3/Baf60c controls
Jordan NV, Prat A, Abell AN, Zawistowski JS, Sciaky N, Karginova OA, Zhou B, Golitz BT, Perou CM, Johnson GL. Mol Cell Biol. 2013 Aug;33(15):3011-25.
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Chang CJ, Chao CH, Xia W, Yang JY, Xiong Y, Li CW, Yu WH, Rehman SK, Hsu JL, Lee HH, Liu M, Chen CT, Yu D, Hung MC. Nat Cell Biol. 2011 Mar;13(3):317-23.