Anti-N Cadherin antibody
| Name | Anti-N Cadherin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab12221 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-F IHC-P |
| Species Reactivities | Mouse, Rat, Chicken, Bovine, Human, Zebrafish |
| Antigen | Synthetic peptide: RMDERPIHAEPQYPVRSAAP , corresponding to amino acids 808-827 of Human N Cadherin |
| Description | Rabbit Polyclonal |
| Gene | CDH2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Paraffin-embedded sections of 15-day mouse embryo. Antigen retrieval: microwave treatment, 3 minutes x 10 times, 10 mM citrate buffer. Primary antibody: 10 ug/ml ab12221, 1 hour, room temperature. Secondary antibody: Envision plus Colorimetric detection: DAB
ab12221 at 1/200 dilution staining rat heart tissue sections by IHC-P. The tissue was fixed with Histochoice and paraffin sectioned prior to staining. An Alexa-Fluor conjugated donkey anti-rabbit antibody was used as the secondary. The image depicts stained N-Cadherin (green) and counterstained nuclei (DAPI-blue) overlay (left panel). The DIC (phase) image of the same tissue, superimposed with fluorescence overlay (right panel) confirms the localization of the N-Cadherin at intercalated discs region. Note, the phase contrast image shows typical uninucleated, cylindrical morphology of cardiac myocytes; that are often branched and joined to each other end-to-end (intercalated discs) to form interlacing network.See Abreview
ab12221, at 1/100, staining N Cadherin in mouse differentiated embryonic stem cells by immunocytochemistry / immunofluorescence. Cells were methanol/acetone (1/1) fixed and permeabilized in 0.1% triton X prior to blocking in 1% serum and 0.1% BSA for 30 minutes at 21°C. Alexa fluor® 488 goat polyclonal to rabbit Ig, diluted 1/200, was used as the secondary antibody.See Abreview
All lanes : Anti-N Cadherin antibody (ab12221) at 1/100 dilutionLane 1 : MDA-MB-231 cells (Negative Control)Lane 2 : U2OS cells (Positive Control)Lysates/proteins at 200000 cells per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-N Cadherin antibody (ab12221) at 1/100 dilutionLane 1 : Whole cell lysate prepared from human glioblastoma cell line U373MGLane 2 : Whole cell lysate prepared from human glioblastoma cell line U251Lysates/proteins at 100 µg per lane.SecondaryHRP monoclonal anti-rabbit immunoglobulins at 1/7500 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of Human lung tissue, with idiopathic pulmonary fibrosis, staining N Cadherin with ab12221. Tissue was fixed in aceteone/methanol (1:1) and incubated with primary antibody overnight at 4°C. An Oregon Green 488-conjugated anti-rabbit IgG was used as the secondary antibody.
Product References
Epithelial-mesenchymal status influences how cells deposit fibrillin - Epithelial-mesenchymal status influences how cells deposit fibrillin
Baldwin AK, Cain SA, Lennon R, Godwin A, Merry CL, Kielty CM. J Cell Sci. 2014 Jan 1;127(Pt 1):158-71.
Loss of Sertoli-germ cell adhesion determines the rapid germ cell elimination - Loss of Sertoli-germ cell adhesion determines the rapid germ cell elimination
Luaces JP, Rossi LF, Sciurano RB, Rebuzzini P, Merico V, Zuccotti M, Merani MS, Garagna S. Biol Reprod. 2014 Mar 6;90(3):48.
Utilization of liquid chromatography mass spectrometry analyses to identify - Utilization of liquid chromatography mass spectrometry analyses to identify
Jian SF, Hsiao CC, Chen SY, Weng CC, Kuo TL, Wu DC, Hung WC, Cheng KH. Mol Cancer Res. 2014 Apr;12(4):622-35.
Zebrafish reporter lines reveal in vivo signaling pathway activities involved in - Zebrafish reporter lines reveal in vivo signaling pathway activities involved in
Schiavone M, Rampazzo E, Casari A, Battilana G, Persano L, Moro E, Liu S, Leach SD, Tiso N, Argenton F. Dis Model Mech. 2014 Jul;7(7):883-94.
A human in vitro model that mimics the renal proximal tubule. - A human in vitro model that mimics the renal proximal tubule.
Hoppensack A, Kazanecki CC, Colter D, Gosiewska A, Schanz J, Walles H, Schenke-Layland K. Tissue Eng Part C Methods. 2014 Jul;20(7):599-609. doi:
Nuclear factor I-C (NFIC) regulates dentin sialophosphoprotein (DSPP) and - Nuclear factor I-C (NFIC) regulates dentin sialophosphoprotein (DSPP) and
Lee HK, Lee DS, Park SJ, Cho KH, Bae HS, Park JC. J Biol Chem. 2014 Oct 10;289(41):28225-36.
Bison and bovine rectoanal junctions exhibit similar cellular architecture and - Bison and bovine rectoanal junctions exhibit similar cellular architecture and
Kudva IT, Stasko JA. BMC Vet Res. 2013 Dec 28;9:266.
Differential stem- and progenitor-cell trafficking by prostaglandin E2. - Differential stem- and progenitor-cell trafficking by prostaglandin E2.
Hoggatt J, Mohammad KS, Singh P, Hoggatt AF, Chitteti BR, Speth JM, Hu P, Poteat BA, Stilger KN, Ferraro F, Silberstein L, Wong FK, Farag SS, Czader M, Milne GL, Breyer RM, Serezani CH, Scadden DT, Guise TA, Srour EF, Pelus LM. Nature. 2013 Mar 21;495(7441):365-9.
Induced pluripotent stem cell-derived cardiac progenitors differentiate to - Induced pluripotent stem cell-derived cardiac progenitors differentiate to
Christoforou N, Liau B, Chakraborty S, Chellapan M, Bursac N, Leong KW. PLoS One. 2013 Jun 13;8(6):e65963.
Stem cell marker nestin is critical for TGF-beta1-mediated tumor progression in - Stem cell marker nestin is critical for TGF-beta1-mediated tumor progression in
Su HT, Weng CC, Hsiao PJ, Chen LH, Kuo TL, Chen YW, Kuo KK, Cheng KH. Mol Cancer Res. 2013 Jul;11(7):768-79.