ab119752 at 1/200 dilution staining N Cadherin (phospho Y860) in Mouse C2C12 cells, treated with pervanadate (right). The cells on the left were not treated with pervanadate prior to analysis. Detection used an appropriate secondary antibody conjugated to Cy3.
Lanes 1 - 2 : Anti-N Cadherin (Cytoplasmic) at 1/1000 dilutionLanes 3 - 4 : Anti-N Cadherin (phospho Y820) at 1/1000 dilutionLanes 5 - 6 : Anti-N Cadherin (a.a. 853-864) at 1/1000 dilutionLanes 7 - 8 : Anti-N Cadherin (phospho Y860) antibody (ab119752) at 1/1000 dilutionLane 1 : Human endothelial cells, untreated Lane 2 : Human endothelial cells, treated with pervanadate (1 mM) for 30 minLane 3 : Human endothelial cells, untreated Lane 4 : Human endothelial cells, treated with pervanadate (1 mM) for 30 minLane 5 : Human endothelial cells, untreated Lane 6 : Human endothelial cells treated with pervanadate (1 mM) for 30 minLane 7 : Human endothelial cells, untreated Lane 8 : Human endothelial cells, treated with pervanadate (1 mM) for 30 min