Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling MRP1 using ab180960 at 1/10 dilution (green) or Rabbit monoclonal IgG (red) followed by Goat anti rabbit IgG (FITC) at 1/150 dilution.
Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960) at 1/5000 dilution + A549 cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960) at 1/2000 dilutionLane 1 : A431 cell lysateLane 2 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution