All lanes : Anti-Monoamine Oxidase B antibody [EPR7103] (ab125010) at 1/5000 dilution (purified)Lane 1 : Mouse brainLane 2 : Rat brainLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-Monoamine Oxidase B antibody [EPR7103] (ab125010) at 1/1000 dilution (unpurified)Lane 1 : Human fetal kidney lysateLane 2 : Human platelet lysateLane 3 : Human fetal brain lysateLane 4 : Human fetal liver lysateLane 5 : 293T lysateLane 6 : A549 lysateLane 7 : SH-SY5Y lysateLane 8 : HepG2 lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling Monoamine Oxidase B with purified ab125010 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling Monoamine Oxidase B with purified ab125010 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Monoamine Oxidase B with unpurified ab125010 at 1/50.

ab125010 (purified) at 1/30 immunoprecipitating Monoamine Oxidase B in HEK293 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

Flow Cytometry analysis of 293T cells labelling Monoamine Oxidase B with purified ab125010 at 1/110 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD