Anti-Mimitin antibody [EP15279] - C-terminal (ab192267) at 1/10000 dilution + Human fetal heart lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-Mimitin antibody [EP15279] - C-terminal (ab192267) at 1/1000 dilutionLane 1 : Mouse heart lysateLane 2 : Mouse kidney lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Mimitin with ab192267 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Mimitin with ab192267 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown.

Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Mimitin with ab192267 at 1/100 dilution. The cells were permeabilised with 0.1% Triton X-100. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/200 dilution was used as the secondary antibody (green). The nuclear counter stain is DAPI (blue). Negative controls, ab192267 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.

Western blot analysis of Mimitin in immunoprecipitation pellets from MDA-MB-435 lysate. ab192267 used at 1/60 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1000 dilution.