ab157210 showing -ve staining in Human normal brain.

ab157210 showing +ve staining in Human normal colon.

All lanes : Anti-MHC Class II antibody [EPR11226] (ab157210) at 1/10000 dilutionLane 1 : Human fetal thymus cell lysateLane 2 : Human tonsil cell lysateLysates/proteins at 10 µg per lane.

Anti-MHC Class II antibody [EPR11226] (ab157210) at 1/10000 dilution + Immunoprecipitation pellet from Human fetal thymus lysate at 10 µg

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MHC Class II with ab157210 at 1/100 dilution.

Immunofluorescent analysis of Jurkat cells labeling MHC Class II with ab157210 at 1/50 dilution.

Overlay histogram showing Raji cells stained with ab157210 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab157210, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.