Anti-MHC Class 1 H2 Db antibody [28-14-8]
| Name | Anti-MHC Class 1 H2 Db antibody [28-14-8] |
|---|---|
| Supplier | Abcam |
| Catalog | ab25228 |
| Prices | $382.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | 28-14-8 |
| Applications | FC IHC-F IHC-P |
| Species Reactivities | Mouse |
| Antigen | C3H |
| Description | Mouse Monoclonal |
| Gene | H2-D1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of MHC Class 1 H2 Db staining in mouse spleen formalin fixed paraffin embedded tissue section, using Mouse-on-Mouse detection kit, ab127055. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. The section was incubated with ab25228, 10µg/ml, for 15 mins at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
![Overlay histogram showing SP0/2 cells stained with ab25228 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25228, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/22450_MHC-Class-1-H2-Db-Primary-antibodies-ab25228-1.jpg)
Overlay histogram showing SP0/2 cells stained with ab25228 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25228, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
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Domain interactions of H-2 class I antigens alter cytotoxic T-cell recognition - Domain interactions of H-2 class I antigens alter cytotoxic T-cell recognition
Allen H, Wraith D, Pala P, Askonas B, Flavell RA. Nature. 1984 May 17-23;309(5965):279-81.
Specific recognition of the product of a transferred major histocompatibility - Specific recognition of the product of a transferred major histocompatibility
Woodward JG, Orn A, Harmon RC, Goodenow RS, Hood L, Frelinger JA. Proc Natl Acad Sci U S A. 1982 Jun;79(11):3613-7.
Product of a transferred H-2Ld gene acts as restriction element for LCMV-specific - Product of a transferred H-2Ld gene acts as restriction element for LCMV-specific
Orn A, Goodenow RS, Hood L, Brayton PR, Woodward JG, Harmon RC, Frelinger JA. Nature. 1982 Jun 3;297(5865):415-7.