All lanes : Anti-Meis homeobox 3 antibody [EPR15416] (ab185961) at 1/5000 dilutionLane 1 : MCF7 cell lysateLane 2 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-Meis homeobox 3 antibody [EPR15416] (ab185961) at 1/5000 dilutionLane 1 : Human fetal lung lysateLane 2 : Human fetal heart lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
All lanes : Anti-Meis homeobox 3 antibody [EPR15416] (ab185961) at 1/1000 dilutionLane 1 : Rat brain lysateLane 2 : Rat heart lysateLane 3 : Rat kidney lysateLane 4 : Rat spleen lysateLane 5 : RAW 264.7 cell lysateLane 6 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow Cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Meis homeobox 3 with ab185961 at 1/210 dilution (red) compared with a rabbit monoclonal IgG isotype control (green). Detection utilised Goat anti rabbit IgG (FITC) at 1/150 dilution.