
Lane 1 : Anti-MC5 Receptor antibody (ab87470) at 1 µg/ml (Blocked in 5% BSA)Lane 2 : Anti-MC5 Receptor antibody (ab87470) at 1 µg/ml (Blocked in 3% MILK)Lane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 2 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lysates/proteins at 10 µg per lane.SecondaryLane 1 : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutionLane 2 : Active rat MCP1 protein fragment (ab9780) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.