
All lanes : Anti-MAPK15 antibody - N-terminal (ab70911) at 1/50 dilutionLane 1 : Nontransfected 293 cell line lysateLane 2 : 293 cell line lysate transiently transfected with the MAPK15 geneLysates/proteins at 2 µg per lane.Observed band size : 70 kDa (why is the actual band size different from the predicted?)

ab70911, at a 1/10 dilution, staining human MAPK15 in breast carcinoma, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue. ab70911 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.